Google Scholar. Ortega, A.; Garca de la Torre, J. 20K. * shows a significant difference between the M.W. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Front Microbiol. Advances in Physical Chemistry, Vol. 10K, and c M.W. 4K and M.W. "Dextrans in Aqueous Solution. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. ; Prasad, D.H.L. Manage cookies/Do not sell my data we use in the preference centre. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Before Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Polysaccharides-based nanoparticles as drug delivery systems. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Gums and stabilisers for the Food Industry, 5, 447-450. Carbohydrate Polymers 95 360-365. Polyelectrolytes as secondary calibration standards for aqueous SEC. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Soc., 21, 1, 78-86. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Drug Deliv Syst. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Journal of Physical Chemistry. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Dextran produced from Weissella cibaria JAG8 was purified and characterized. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. (2007). Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Prausnitz MR, Langer R. Transdermal drug delivery. Pharmacol Pharm. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Solvent effects on polysaccharide conformation. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Nat Biotechnol. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. The Viscosity of Dilute Solutions of Long-Chain Molecules. 113, 81-86. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Masuelli, M. A. Die Makromolekulare Chemie, 148(1), 229-238. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Biomaterials. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. All authors read and approved the final manuscript. Google Scholar. Format), Citation-(EndNote The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). 2006;3(3):37385. Microporation applications for enhancing drug delivery. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Molecular weight, molecular weight distribution and molecular size of a native dextran. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. (2013). Exp Cell Res. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Fixable: Fixable via Lysine. 2015;23(3):2239. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. CAS Journal of American Chemical Society, 64(11), 2716-2718. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. Journal Non-Newtonian Fluid Mech., 97, 295-301. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. 2019;9(3-S):9726. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Webof overall macromolecular geometry and hydrodynamic draining. Differences were considered statistically significant at p <0.05. Progress in Polymer Science, 30, 38-70. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. ; Patel, T.R. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. Pathan IB, Setty CM. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Dextrans can function efficiently as anterograde or retrograde tracers, Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Masuelli, Martin Alberto (2013). The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. European Biophysical Journal, 36, 85-94. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Trop J Pharm Res. A solvent at this temperature is called a theta solvent [53]. The slope is a value and intercept is equal to the ln kMH value [47]. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. Materiaux 1-5. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. ; De Jong, S.J. Kumar SV, Tarun P, Kumar TA. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. The solution was measured using an Anton Paar DMA35N densitometer. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Optical properties of dextran in solution and in films. Intrinsic viscosity of bovine serum albumin conformers. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. 2011;16(2):518. The Mark-Houwink. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. All authors have consented to the submission of this manuscript for publication. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. H&E stained images were analyzed using Image J software. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. Clipboard, Search History, and several other advanced features are temporarily unavailable. Polymer molecular weight distribution. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Springer Editions. Kasaai, Mohammad R. (2007). Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Food Funct. Biomater Res 24, 22 (2020). Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. Sci. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. CAS WebR H Hydrodynamic radius [m] The basic DLS setup. Ahn, G.Y., Eo, HS., Kim, D. et al. (1969). ACS Nano. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol ; Rjumtsev, E.I. Gekko, K. (1971). Anal. Correspondence to These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. Journal of American Chemical Society, 76 (20), 5041-5052. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. dextran hydrodynamic radius. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Global hydrodynamic analysis of the molecular flexibility of galactomannans. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Not for use in diagnostic procedures. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Treatment of acne scars by fractional bipolar radiofrequency energy. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Guner, Ali (1999). Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Carbohydrate Polymers, 74, 372-378. Macromolecules, 29, 2265-2268. (2006). 2012;101(8):28619. Rotureau, E.; Dellacherie, E.; Durand, A. Journal of Chemical Education, 49(8), 577-579. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. (2003). @ Nilimesh See attached. 2019;26(1):25261. (1999). Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. 8. 1905/05) for their financial support. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Disclaimer. Carbohydrate Research, 340, 811-826. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. This site needs JavaScript to work properly. Carbohydrate Polymers, 39, 377-380. On the molecular weight distribution of dextran T-500. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. Microporation was performed 2 times on each sample. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Ind. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). ; van Nostrum, C.F. Corp. (Seoul, Korea). Webdextran hydrodynamic radius. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. (2013). The micropores of the human cadaver skin were regenerated according to the time. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Synthesis and characterization of blue dextrans. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Hennink, W.E. Expert Rev Med Devices. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). Advances in Carbohydrate Analysis, 1, 63-144. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Thermo Fisher Scientific. Google Scholar. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Harding, Stephen E. (2005). The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. official website and that any information you provide is encrypted Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. 43-1, 115-147. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. Study of temperature influence. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). (1999). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth (1987). Gold MH, Biron JA. 4K, b M.W. Webwhittier union high school district superintendent. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. Dextran Fractions. Learn More. ; Ross-Murphy, S.B. Dilute Solution. De Belder, A.N. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ].